In American MacArthur foundation as the first "genius award" Chinese female scientists, ZhuangXiaoWei professor received many important achievements, especially in biophysics microscopic imaging fields, professor in the cells ZhuangXiaoWei recently published entitled "Super - BreakingtheDiffractionBarrier: ResolutionImagingofCells," describes the Super resolution of the latest progress cell image.
The traditional optical microscope the wavelength of the light, for limited 200nm following thing can only shake head were left feeling useless. Although the electronic microscope can achieve nanoscale resolution, but the results easy to cause the sample energized the destruction of the samples, so can observation is also limited. Molecular biologists although can do some want to observe the protein labeled fluorescence volume label, but these proteins or often crowded at a cake of, under the microscope can't tell who's who.
This years high resolution across a big step fluorescence microscope, allows researchers to the nanometer level observation cells from prominence, thus declared stretch -- 750 nano size range of 200 fuzzy mass times over. Like using photosensitive positioning microscope: can be used to observe the PALM nanoscale biological, compared with electron microscope has more clear contrast, if give different protein grafted in different fluorescent marker, it can be used to further protein-protein interactions.
ZhuangXiaoWei research team has been studying how to use photosensitive switch probe to achieve single molecule luminous technology. They hope can photosensitive switch will originally overlap few molecular image so that it can temporarily apart, and the single molecule images obtained, so as to improve the resolution.
ZhuangXiaoWei team in 2004 found by chance some flower green dye with optical switch, that is, through the use of a different color of light, can casually turn them into fluorescence state and activate deactivation into the dark state. Since then ZhuangXiaoWei born began to study these optical probe, use them to a brief separation individual molecules in space images so as to improve the resolution overlapping.
In the NatureMethods after this journal articles, naming a random optical microscope (stochasticopticalreconstructionmicroscopy, STORM reconstruction. Use the resolution of the 20nm STORM can see with DNA molecules and DNA - protein complex molecules. This method is based on the photonic controllable switching fluororescent probes and centroid localization theory, under the dual laser stimulate shine, and through random fluorospectrophotometric probes molecular orientation and molecular location overlap of ultra-high resolution image reconstruction formation, the spatial resolution currently can reach 20nm. STORM although can provide higher spatial resolution, but imaging time often needs a few minutes, also can not meet the real-time visual imaging liing space greatly, development needs.
Recent ZhuangXiaoWei research group in Science magazine also published their 3DSTORM imaging results, this technology spatial resolution than ever before in all optical 3D imaging technology to resolution 10 times higher. The researchers showed 3DSTORM imaging technology with filmed renal cell inside microtubules chart and other molecule structure. Later, they bring the technology development and further into multiple color 3D imaging technology (multicolor3Dimaging).
In addition, another group ZhuangXiaoWei outside Chinese research team in this respect has also won the outstanding achievement, XieXiaoLiang from Harvard University's professor in a single molecular spectroscopy testing and its application in life science also made many contribution, ten years ago, professor XieXiaoLiang because released CARS microscopy techniques and sparked huge sensation. This kind of technology through an enzyme called spontaneous Raman scattering phenomenon to enhance signal. In the spontaneous Raman scattering, the chemical bonds can change within the sample by one of the light wave length. The more early use of Raman scattering microcopy requirements of the laser power very tall, and sometimes need exposure lasted one day.
Recent XieXiaoLiang professor team will SRS microscopy techniques and nuclear magnetic resonance imaging (MRI) technology, which can unite the rapid sensitive molecular motion capture living tissue, such as blood extrusion through the process of blood vessels.
The technology level sub-cellular lens resolution, can record levels of protein, fat, and cells of the fluid inside. Due to the SRS microscope is able to detect the chemical bonds resonance between atoms, so that no fluorescent marker. Researchers believe that the SRS microscope in tumor excision surgery can help to accelerate in operation process. The traditional sample analysis takes about 20 minutes, SRS microscope can almost achieves real-time scanning.
With several common observation of biological molecules, compared to new SRS technical advantages microscopy: it can illuminate the biological sample collection and analysis of laser, nearly 30 percent higher than the traditional SRS microscope 30 times; And don't need to insert fluorescent marker, to avoid the green fluorescent marker protein disrupt biological path or pinned smaller biological molecules. In addition, the traditional infrared microscopy is too low, and spatial resolution to sample dehydration; need Natural Raman microscopic need high laser energy, whole time-consuming very long, the application in living samples is restricted; Raman scattering coherent anti stowe carat microscopy in the lipid filmed outside except when most molecules, and there is not enough contrast new SRS microscopy techniques can break through these limitations.